CHS

Cholesteryl Hemisuccinate Tris Salt

Packs & prices

CHS packaging and prices in USD and EUR
Pack size	Price (USD / EUR)	Enquire
1 gDC-R0002-1G	$48 / €43
5 gDC-R0002-5G	$189 / €169
25 gDC-R0002-25G	$716 / €639

1 gDC-R0002-1G
$48 / €43
5 gDC-R0002-5G
$189 / €169
25 gDC-R0002-25G
$716 / €639

Notice: Prices are per pack in USD / EUR. Enquire to confirm availability, lead time, shipping costs and applicable taxes. Contact us for bulk or recurring supply.

Product overview

CHS Tris salt combines a cholesterol-derived hydrophobic scaffold with a hemisuccinate polar group and Tris counterion. It is typically used with a primary detergent, rather than as the sole membrane protein solubilizing agent. The supplied application data demonstrate its combined use with LMNG in GPCR extraction, purification and structural studies.

Product specifications

CAS number: 102601-49-0
Molecular formula: C₃₅H₆₁NO₇
Molecular weight: 607.86 g/mol
Product form: Cholesteryl hemisuccinate Tris salt; verify this salt form against your protocol.
Appearance: White solid
Purity specification: ≥ 99.0%
Solubility in CHAPS solution: 2.5% in 6% aqueous CHAPS, with ultrasound-assisted dissolution
Solubility in DDM solution: 0.8% in 10% aqueous DDM, with ultrasound-assisted dissolution
Handling: Store at low temperature and protect from moisture.
Storage: Powder: −20 °C, up to 3 years. In solvent: −80 °C, up to 1 year, according to the technical sheet.

Identity and analytical characterization

IR, NMR and representative HPLC / ELSD results for the supplied CHS Tris salt.

CHS Tris salt infrared spectrum — **IR spectrum**Representative IR record for CHS Tris salt.

CHS Tris salt proton NMR spectrum — **¹H NMR spectrum**Representative ¹H NMR record for CHS Tris salt.

CHS Tris salt HPLC and ELSD chromatograms — **HPLC / ELSD analytical record**Sample CHS-035-P-3 · UV / ELSD · 10 μL injection.

HPLC peak integration · CHS-035-P-3

HPLC peak integration · CHS-035-P-3
Peak	RT (min)	Area	Height	Height (%)	Area (%)
1	2.691	7,957,674	703,039	99.554	99.893
2	3.191	5,146	1,815	0.257	0.064
3	3.444	134	83	0.012	0.002
4	3.723	1,234	412	0.058	0.015
5	4.583	1,421	560	0.079	0.018
6	5.140	531	224	0.032	0.007
7	12.574	82	56	0.008	0.001
Total	—	7,984,221	706,190	100.000	100.000

LMNG / CHS membrane protein validation

Application data for LMNG and CHS used together. These results describe the tested receptor preparations and do not represent a universal outcome for every membrane protein.

SEC-HPLC chromatograms of hNTSR1 and A₂AR — **SEC-HPLC of purified hNTSR1 and A₂AR**Predominant main peaks with minor aggregate peaks.

SDS-PAGE gel of purified hNTSR1 and A₂AR — **SDS-PAGE of purified GPCRs**hNTSR1 and A₂AR bands at the expected molecular weights.

Cryo-EM maps of A₂AR–Gs and GCGR–Gs — **Cryo-EM structure examples**LMNG / CHS preparations: A₂AR–Gs, 2.86 Å; GCGR–Gs, 3.69 Å.

Handling & use

Select working concentrations for the target protein and detergent system.

Representative analytical records are not a batch-specific certificate of analysis.