D-Luciferin Potassium Salt

Bioluminescence imaging and luciferase assay substrate

Packs & prices

D-Luciferin Potassium Salt packaging and prices in USD and EUR
Pack size	Price (USD / EUR)	Enquire
100 mgDC-R0003-100MG	$94 / €84
1 gDC-R0003-1G	$281 / €251
5 gDC-R0003-5G	$1,123 / €1,002

100 mgDC-R0003-100MG
$94 / €84
1 gDC-R0003-1G
$281 / €251
5 gDC-R0003-5G
$1,123 / €1,002

Notice: Prices are per pack in USD / EUR. Enquire to confirm availability, lead time, shipping costs and applicable taxes. Contact us for bulk or recurring supply.

Product overview

D-Luciferin potassium salt is a water-soluble form of the luciferase substrate D-luciferin. In ATP-dependent luciferase reactions, substrate oxidation produces bioluminescence. Applications include reporter assays and compatible in vitro or in vivo imaging workflows. The product-specific activity data presented here are from plasmid-transfected HEK293 cells; animal imaging conditions require protocol-specific evaluation.

Product specifications

CAS number: 115144-35-9
Molecular formula: C₁₁H₇KN₂O₃S₂
Molecular weight: 318.41 g/mol
Chemical name: (S)-4,5-Dihydro-2-(6-hydroxybenzothiazol-2-yl)thiazole-4-carboxylic acid potassium salt
Appearance: Light yellow solid
Purity specification: ≥ 99.0%
Water solubility: 30 mg/mL (technical sheet)
PBS preparation: Confirm working concentration and dissolution conditions for your buffer and protocol with technical support.
Handling: Store at low temperature and protect from moisture.
Storage: Powder: −20 °C, up to 3 years. In solvent: −80 °C, up to 1 year, according to the technical sheet.

Identity and purity characterization

Representative NMR and HPLC analytical records. The peak-area values describe the stated sample and measurement method.

D-luciferin potassium salt proton NMR spectrum — **¹H NMR spectrum**Representative ¹H NMR record for D-luciferin potassium salt.

D-luciferin potassium salt HPLC chromatogram at 220 nm — **HPLC at 220 nm**Sample D-LP-018-1 · 220 nm · 2 μL injection.

HPLC peak integration · D-LP-018-1

HPLC peak integration · D-LP-018-1
Peak	RT (min)	Area	Height	Height (%)	Area (%)
1	5.478	17,649	2,568	0.120	0.092
2	6.219	19,255,669	2,128,710	99.855	99.884
3	6.571	458	410	0.019	0.002
4	7.138	4,242	121	0.006	0.022
Total	—	19,278,018	2,131,809	100.000	100.000

LC-MS characterization

Sample D-LP-018-1-P; injection 1 μL; vial 1-49. Method AM-LCMS01-FA (5 min, mass range 100–1000); batch RXY-260708-1.lcb. Acquired July 8, 2026, 10:00:15; refreshed July 9, 13:03:12. Analyst / processor: Organic Synthesis Department.

D-luciferin potassium salt UV and total-ion chromatograms — **UV and total-ion chromatograms**UV: 220 / 254 nm · Positive- and negative-ion TIC.

D-luciferin potassium salt positive- and negative-ion mass spectra — **Positive- and negative-ion mass spectra**Positive-ion: 2.721 min · Negative-ion: 2.725 min.

LC-MS UV peak integration

LC-MS UV peak integration
Detector	Peak	RT (min)	Area	Height	Height / area (%)
220 nm	5	2.761	4,187,213	1,242,118	100.000 / 100.000
254 nm	5	2.760	957,497	281,622	100.000 / 100.000

Cell-based bioluminescence activity

HEK293 cells transfected with the MA0509 plasmid were measured using a microplate reader after addition of D-luciferin potassium salt working solutions. The supplied assay compares D-LP and a reference product at matched concentrations.

Cell system: MA0509-transfected HEK293
Working concentrations: 50, 150, 500 and 1000 μg/mL
Acquisition: 100 readings per time course

Bioluminescence time courses for D-LP and a reference product — **Bioluminescence kinetics**D-LP and reference product showed similar luminescence profiles under the tested conditions.

Handling & use

Choose working concentration and measurement timing for the luciferase system and sample model.
The supplied cell assay is not a product-specific animal imaging validation or an in vivo-grade certification.

Representative analytical records are not a batch-specific certificate of analysis.